FIG 5.

(A) Regrowth following starvation of the P. aeruginosa Δhpf strain containing hpf with random point mutations obtained by error-prone PCR and screening for a resuscitation defect and the P. aeruginosa Δhpf strain containing the vector control. (B) Regrowth following starvation of the P. aeruginosa Δhpf strain complemented with hpf where the mutations shown in panel A were replaced by alanine. (C) Resuscitation of P. aeruginosa Δhpf strains containing point mutations, enumerated as CFU over 5 days of starvation. Shown are the responses of hpf with mutations obtained by random mutagenesis and screening for resuscitation defect (dashed lines) and mutations with alanine in equivalent positions as the random mutations (solid lines). (D) The 23S to 16S rRNA ratios of the P. aeruginosa Δhpf strain with mutant hpf alleles prior to starvation and following 4 days of starvation. ***, significant difference at P < 0.0001.