FIG 7.

Resuscitation and ribosome protection of the P. aeruginosa Δhpf strain and the Δhpf Δrmf double mutant containing hpf truncated to 95 amino acids. (A) Resuscitation over the course of starvation determined as time to reach an OD of 0.3 for the Δhpf mutant with vector control, the Δhpf Δrmf double mutant with vector control, and complemented strains. The hpf95 truncated gene complemented both the Δhpf mutant and the Δhpf Δrmf double mutant. (B) Ribosome protection of truncated hpf95 in the Δhpf mutant and in Δhpf Δrmf double mutant, measured as 23S/16S rRNA ratio prior to starvation and after 4 days of starvation. ***, significant difference at P < 0.0001.