| Antibodies |
| Rabbit anti RFP |
Rockland |
600-401-379 |
| Mouse anti mcherry |
Living Colors |
632543 |
| Mouse anti MF 20 |
Developmental Studies Hybridoma Bank |
RRID; AB_2147781 |
| Mouse anti Collagen II |
Developmental Studies Hybridoma Bank |
II-II6B3 |
| Mouse anti myosin heavy chain (MHC) |
Developmental Studies Hybridoma Bank |
A4.1025 |
| Rabbit anti GFP |
ThermoFisher Scientific |
A-21311 |
| Phospho-Smad1/5 |
Cell Signaling Technology |
9516T |
| Goat anti-Rabbit Alexa Fluor 488 |
ThermoFisher Scientific |
A-11008 |
| Goat anti-Mouse Alexa Fluor 488 |
ThermoFisher Scientific |
A-21121 |
| Goat anti-Rabbit Alexa Fluor 594 |
ThermoFisher Scientific |
A-11012 |
| Goat anti-Mouse Alexa Fluor 647 |
ThermoFisher Scientific |
A-21241 |
| Donkey anti-Rabbit Alexa Fluor 647 |
abcam |
ab150067 |
| Chemicals, Peptides, and Recombinant Proteins |
|
N-Phenylthiourea |
Sigma |
P7629 |
| DMSO |
Sigma |
276855 |
| Metronidazole |
Sigma |
M3761 |
| (Z)-4-Hydroxytamoxifen |
Sigma |
H7904 |
| Dorsomorphin |
Cayman Chemical |
866405-64-3 |
| LDN-193189 |
Cayman Chemical |
1062368-62-0 |
| Experimental Models: Organisms/Strains |
| scxa:gal4-vp16 |
This study |
N/A |
| uas:creERT2 |
This study |
N/A |
| uas:epNTR-RFP |
[28] |
y268Tg |
| uas-E1b:Kaede |
[93] |
s1999tTg |
| ubi:Zebrabow |
[43] |
a131Tg |
| uas:Eco.Nsfb-mcherry |
[94] |
rw0144Tg |
| sox10:ERT2-Cre |
[44] |
zf760Tg |
| col2a1a:eGFP |
[95] |
nu13Tg |
| scxa:mcherry |
Galloway lab, MGH, USA |
fb301Tg |
| mylz2:Amcyan |
[96] |
N/A |
| mylz2:mcherry |
[96] |
N/A |
| tp1:eGFP |
[54] |
um14Tg |
| shha:eGFP |
[57] |
sb15Tg |
| otm:d2eGFP |
[55] |
kyu1Tg |
| bre:eGFP |
[56] |
pt510Tg |
| hsp70:nog3 |
[97] |
fr14Tg |
| hsp70:bmp2b |
[97] |
fr13Tg |
| drl:creERT2 |
[45] |
N/A |
| runx2:eGFP |
[72] |
zf259Tg |
| osc:eGFP |
[72] |
hu4008Tg |
| nkx2.5:ZsYellow |
[98] |
fb7Tg |
| nxk2.5:ERT2CreERT2 |
[46] |
fb8Tg |
| Tübingen |
Galloway lab, MGH, USA |
N/A |
| Oligonucleotides |
| Primers, see Table S1–S3
|
This paper |
N/A |
| Recombinant DNA |
| 10xuas-creERT2-polyA |
This paper |
N/A |
| scxa-gal4-vp16 |
This paper |
N/A |
| Software and Algorithms |
| ImageJ |
Galloway lab, MGH, USA |
N/A |
| GraphPad Prism 5 |
Galloway lab, MGH, USA |
N/A |
| Adobe Photoshop CS6 |
Galloway lab, MGH, USA |
N/A |
| Microsoft Excel |
Galloway lab, MGH, USA |
N/A |
