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. Author manuscript; available in PMC: 2020 Sep 11.
Published in final edited form as: J Am Soc Mass Spectrom. 2020 Aug 4;31(9):1854–1860. doi: 10.1021/jasms.0c00110

Figure 1. Details of 12-plex DiLeu labeling.

Figure 1.

A: Precursor ion of DiLeu-labeled citrulline; B: An MS2 spectrum of the 12-plex DiLeu-labeled citrulline acquired in the Orbitrap at 60 K resolving power. Low m/z region showing distinct DiLeu reporter channels (bottom) and after zooming in, twelve distinct reporter ion peaks are present (bottom); C: Citrulline changing trends from different time points of three biological replicates. D: Sample labeling map showing the 12-plex DiLeu tags and time point (randomized)