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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Cell Chem Biol. 2020 Jul 9;27(8):1084–1096.e4. doi: 10.1016/j.chembiol.2020.06.014

Figure 6. Parallel Chemoselective Profiling Beyond Cys.

Figure 6.

(A-D) Mutational effect scores for Cys substitutions (Table 1) mapped on the crystal structures of ubiquitin, PTEN, Src kinase domain, and BRCA1 BRCT domain (PDB: 1UBQ, 1D5R, 2SRC and 1T29, respectively). (E,G,I,K) Fractions of all Met mutants classified as WT-like. (F,H,J,L) Fraction of all WT-like Met substitutions that are solvent exposed in each crystal structure. (M) All single amino acid substitutions from the DMS of Src kinase domain and the fraction of those that result in WT-like substitutions. (N) Chemoselective labeling reagents for Met, Tyr, and Lys. (O) WT-like Met, Lys or Tyr residues mapped with WT-like Cys residues.