Table 1:

Large-scale mutagenesis datasets used in this study.

Data set	Variant length (#AA)	Mutagenized Residues*	Number of Mutations	Mutational completeness** (%)	Selected phenotype	Citation
Ubiquitin	76	2–76	4,800	99	Yeast growth	Roscoe, et al. 2013
PTEN	403	14–351	4,112	54	Protein abundance	Matreyek, et al. 2018
SRC	536	270–519	3,506	73	Kinase activity	Ahler, et al. 2019
BRCA1	1,863	1,649–1,859	1,317	36	HAP1 cell growth	Findlay, et al. 2018

^*Residues represented in our analysis in Figures 2 and 6

^**Proportion of observed mutations relative to all possible single amino acid mutations in mutagenized region in our analyses