KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Phospho-Src Family (Tyr416)	Cell Signaling Technology	Cat#2101
Non-phospho-Src (Tyr416)	Cell Signaling Technology	Cat#2102
Bacterial and Virus Strains
E. coli (BL21 DE3 +GroEL + YopH)	Ahler, et al. 2019
NEB 5-alpha Competent E. coli (high efficiency)	N.E.B.	Cat#C2987H
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Pierce retention time calibration mix	ThermoFisher Scientific	Cat#88321
Isotopically heavy iodoacetamide	Sigma	Cat#721328
Thermolysin	Promega	Cat#V4001
Glu-1-Fibrino (GluFib) peptide	Sigma	CAS#103213-49-6
ULP1*	Brigham, et al. 2013
Critical Commercial Assays
Deposited Data
Ubiquitin crystal structure	Vijay-Kumar, et al. 1987	PDB: 1UBQ
PTEN crystal structure	Lee, et al. 1999	PDB: 1D5R
Src Kinase Domain crystal structure	Azam, et al. 2008	PDB: 3DQW
Src 3D crystal structure	Xu, et al. 1999	PDB: 2SRC
BRCA1 BRCT domain crystal structure	Shiozaki, et al. 2004	PDB: 1T29
Experimental Models: Cell Lines
FreeStyle 293F	ThermoFisher Scientific	Cat#R79007
Experimental Models: Organisms/Strains
Oligonucleotides
DNA oligos	This paper	See Supplemental Item DNA_Oligos_and_Constructs.xlsx
Recombinant DNA
Various point mutants in pCDNA5	This paper	See Supplemental Item DNA_Oligos_and_Constructs.xlsx
Src3D (residues 87–536) in pMCSG7	Ahler, et al. 2019
Software and Algorithms
PyMOL	Schrödinger	https://pymol.org/
GraphPad Prism	GraphPad	https://www.graphpad.com/scientific-software/prism/
Image Studio Light	LiCor	https://www.licor.com/bio/products/software/image_studio_lite/
msConvertGUI	ProteoWizard	http://proteowizard.sourceforge.net/tools.shtml
Skyline	MacCoss Lab (UW)	http://skyline.ms
Other