Figure 6.

Prx1 expression pattern in isolated BMP2^cKO/cKO endosteal cells.

(A) mRNA expression levels quantified by qRT-PCR of CXCL12 and Prx1 in isolated endosteal cells from BMP2^cKO/cKO (KO) and control (Con) cultured under undifferentiated conditions (Day 0) or osteogenic differentiation conditions (Day 7, Day 14 and Day 21 of culture). See in the Materials and Methods section for more details. Data are reported as mean ± SD. of triplicate repeats from n=6 samples normalized to day 0 control. *, p < 0.05, **, p < 0.01, ***, p < 0.001, compared to day 0 control by one-way ANOVA and Sidak’s multiple comparison test. #, p < 0.05, ##, p < 0.01, ###, p < 0.001, compared to Con by unpaired two-tail t-test. (B) Schematic representation of AMD3100 treatment in endosteal cells isolated from BMP2^cKO/cKO and control mice and cultured under osteogenic differentiation conditions, with or without 400 μM AMD3100 every 3 days beginning on day 7 through day 14. See in the Materials and Methods section for more details. (C) mRNA expression levels quantified by qRT-PCR of CXCL12 and Prx1 in isolated endosteal cells from BMP2^cKO/cKO (KO) and control (Con) cultured with or without AMD3100 (as schematically presented in B). Data are reported as mean ± SD. of triplicate repeats from 6 separate samples normalized to day 14 control. *, p < 0.05, compared to control by one-way ANOVA and Sidak’s multiple comparison test. ##, p < 0.01, compared to KO by unpaired two-tail t-test.