Figure 5.

SRD5A1 activates NF-κB/VEGF signaling pathway in CRC cells. (A) Classification of the 20 most possible pathways analyzed by transcriptomic RNA-sequencing [HCT116 knockdown (KD) vs. HCT116 KD + Dox]. Y-axis indicated the name of the pathway, and the X-axis represented the Rich factor. Point size demonstrated the number of differentially expressed genes in one pathway, and the color of the point corresponded to the range of the P-value (B–D). Western blot analysis validated that SRD5A1 KD or inhibition by dutasteride remarkably abrogated expression of NF-κB p65 (B) and p-p65 (C), along with the quantitative results (D). (E) Western blot analysis showed the reductive expression of VEGF with SRD5A1 inhibition. (F) Real-time PCR indicated the significantly downregulated expression of TNF-α and VEGF, followed by SRD5A1 inhibition. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical analysis of each group is compared with the control group (KD/WT). Data were expressed as mean ± SD. Experiments were repeated three times.