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. Author manuscript; available in PMC: 2021 Jan 6.
Published in final edited form as: Nat Microbiol. 2020 Jul 6;5(9):1158–1169. doi: 10.1038/s41564-020-0749-2

Extended Data Fig. 8. Quantification of defective interfering RNA content in virus stocks.

Extended Data Fig. 8

Defective interfering RNA content for A) PB2, B) PB1 and C) PA segments was determined by ddPCR using primer pairs targeting terminal and internal portions of each polymerase gene segment to determine their absolute copy number and produce a ratio of terminal:internal copies. All virus stocks used in this study contained low DI content (terminal:internal ratio less than or equal to 2). A DI-rich control virus, Pan99wt P3 (A/Panama/2007/99 [H3N2]), is included for comparison. This virus stock was passaged three times in MDCK cells at high MOI. For the MaMN99-GFHK99 chimeric viruses, the segments derived from GFHK99 virus are listed in place of the full strain names.