Extended Data Fig. 9. Example gating for flow cytometry to evaluate HA positive cell numbers.

Plots shown were generated in the course of experiments reported in Figure 1 and are representative of results obtained in at least three independent experiments. Following staining for cell-surface HA protein: 1) A population of cells was selected by gating out cell debris by SSC-A vs FSC-A. 2) Multiplets were excluded by gating for single cells in SSC-H vs SSC-W. 3) Populations of infected cells were gated based on expression of the appropriate epitope tag.