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. Author manuscript; available in PMC: 2021 Sep 8.
Published in final edited form as: Circulation. 2020 Jul 2;142(10):948–962. doi: 10.1161/CIRCULATIONAHA.119.044893

Figure 6. CIP4-dependent Ca2+ compartmentation.

Figure 6.

A. Ca2+ FRET biosensors. D3cpv Cameleon (Cam) contains ECFP, mutant calmodulin (mCaM) and CaM-binding peptide from smooth-muscle myosin light-chain kinase, and cp-Venus.39 B. Myocytes expressing sensors were stimulated with Ang II (100 nmol/L). Sensor responses compared by Mann-Whitney Test. C. Myocytes expressing sensors and PP-BFP were paced at 1 Hz. Representative tracings and amplitude change for FRET ratio (R/R0) are shown. Bar graphs show results from individual tracings and mean ± s.e.m. Cam and Cam-CIP4 were compared by Mann-Whitney Test. Data on graph on right were analyzed by Kruskal-Wallis Test followed by Dunn’s Post-hoc testing. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s. – not significant.