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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Dev Biol. 2020 Jul 19;465(2):119–129. doi: 10.1016/j.ydbio.2020.06.012

Figure 4. Expression of corneal endothelium genes during normal ocular development and by the injected pNC.

Figure 4.

Section in situ hybridization was performed on E3, E5, and E7 injected eyes. (A, D, G, J) E3 chick eyes showing little or no expression of RALDH2, RHOB, TGFβ2 and WNT9A in the periocular region. (B, E, H, K) E5 chick eyes robust expression of RALDH2, RHOB, TGFβ2 and WNT9A by the corneal endothelium (arrowheads). (C, F, I, L) E7 chick eyes showing strong expression of expression of RALDH2, RHOB, TGFβ2 and WNT9A by QCPN-positive cells 2 days post injection. Inserts are higher magnifications of selected regions. ec, ectoderm; en, corneal endothelium; ep, corneal epithelium; L, lens; oc, optic cup; str, corneal stroma. Scale bars represent 100 μm (A-L) and the scale bars for the inserts represent 20 μm (C, F, I, L).