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Fluorescence intensity of the nanopore tip-side compartment solution before and after 3-h eGFP translocation in the modified PET nanopore. The experiment was performed at +800 mV with 100 ng/mL of eGFP added to the base side of the modified nanopore bathed in a pH 5.0 electrolyte solution comprising 1 M KCl and 10 mM Tris. The fluorescence spectrum was obtained with λex/em = 488/507 nm at room temperature.
