KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Digoxigenin-POD Fab fragment	Sigma-Aldrich	11207733910
Bacterial and Virus Strains
N/A
Biological Samples
N/A
Chemicals, Peptides, and Recombinant Proteins
Agarose, Type II-A, Medium EEO	Sigma-Aldrich	A9918
Penicillin-Streptomycin 100x	Gibco	15-140-122
TDO Inhibitor 680C91	Sigma-Aldrich	SML0287
Dimethysulfoxide (DMSO)	Sigma-Aldrich	D8418
Diethyl pyrocarbonate	Sigma-Aldrich	D5758
Paraformaldehyde	Electron microscopy sciences	15714
2X NLS Cas9 protein	Synthego
Formamide	Sigma	47670–2.5L-F
DIG wash and block set (Roche blocking buffer)	Sigma	11585762001
RNA from yeast	Sigma	10109223001
SP6 RNA polymerase	Roche	M0207L
DIG-labeling nucleotide mix	Roche	11277073910
Fluoromount-G	Southern biotech	0100–20
Ethanol	Pharmco	111000200
Critical Commercial Assays
pGEM t Easy vector	Promega	A1360
Cy5 TSA plus kit	Perkin Elmer	NEL752001KT
Monarch DNA Kit	NEB	T3010L
Monarch Gel Extraction kit	NEB	T2010L
Deposited Data
TDO locus of Doryteuthis pealeii	GenBank	MT648678
Doryteuthis pealeii RNAseq data	Bioproject	PRJNA641326
Experimental Models: Cell Lines
N/A
Experimental Models: Organisms/Strains
Adult Doryteuthis pealeii	Wild caught in the Vineyard Sound near Woods Hole
Oligonucleotides
In situ PCR primer 1	IDT	GAGCAATCGCGTCAAGTACA
In situ PCR primer 2	IDT	GGCGTTGTCTTCAGGGTAGA
sgRNA 1 PCR forward primer nested reaction 1	IDT	GCCTCAAAACAACCCATATTATTGAGG
sgRNA 1 PCR reverse primer nested reaction 1	IDT	GAGTTGTAGCGCATCTGAGCAC
sgRNA 1 PCR forward primer nested reaction 2	IDT	TAAATACTTGTGTTCATAGGGTACAC
sgRNA 1 PCR reverse primer nested reaction 2	IDT	GGTAAACCCGCTCTGAGTTATTTCC
sgRNA 2 PCR forward primer nested reaction 1	IDT	GCGTGCTATTCTGCATTAGCAC
sgRNA 2 PCR reverse primer nested reaction 1	IDT	CGTTAAACCAGTTCTGCCCTCAAG
sgRNA 2 PCR forward primer nested reaction 2	IDT	CCCTAACCATAACCTTAACGTCTC
sgRNA 2 PCR reverse primer nested reaction 1	IDT	GCATTCTGTACGATGACACTAAGC
CRISPR sgRNAs
sgRNA1	Synthego	CAUCCAAUCAGUGCCGAAGC
sgRNA2	Synthego	UGGCAGCUGAGGUUCGUGUU
Software and Algorithms
MUSCLE	(39)
Fig tree Software		http://tree.bio.ed.ac.uk/software/figtree
Fast tree 2 software	(40)	http://www.microbesonline.org/fasttree/
Star v. 2.7.0	(42)
Bowtie2	(44)
SAMtools	Genome Research Limited	http://www.htslib.org/
Indel.py	This study	https://github.com/pipedq/CRISPR_G0_Genotyping
Cumulative.py	This study	https://github.com/pipedq/CRISPR_G0_Genotyping
Event_frequency.py	This study	https://github.com/pipedq/CRISPR_G0_Genotyping
Other
Quartz glass capillaries for making injection needles	Sutter Instruments	QF100-70-10