Figure 1. AMs are essential and sufficient for airway clearance of S. pneumoniae.
(A) The mean frequency of BALF AMs (CD11c+Siglec-F+), and (B) lung bacterial burdens (mean±SD) 24 h after Spn infection of B6 WT and Csf2rb−/− mice (n ≥ 5 mice/group). Csf2rb−/−+AM: Csf2rb−/− mice received adoptive transfer of WT AMs. **, P<0.01, Mann Whitney test. (C) The mean frequency of BALF CD11c+ and CD11b+ myeloid cells, and (D) lung bacterial burdens (mean±SD) 24 h after Spn infection of BALB/c WT, Rag2−/−Il2rg−/− and hIl3/Csf2 KI mice (n ≥ 5 mice/group). **, P<0.01, Mann Whitney test. (E) Animal survival after i.n. or i.p. challenge of B6 WT mice with 105 CFU Spn. Mice were treated anti-Gr1 antibodies (α-PMN) to deplete PMNs. **P< 0.01, log-rank test. Data shown are representative of at least two independent experiments.