Skip to main content
. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: J Immunol. 2020 Aug 12;205(6):1601–1607. doi: 10.4049/jimmunol.2000094

Figure 4. Temporary AM depletion decreases neutrophil recruitment and promotes bacterial outgrowth in CD169-DTR mice during influenza/S. pneumoniae coinfection.

Figure 4.

(A) The numbers of BALF AMs (CD11c+Siglec-F+), and (B) lung bacterial burdens 24 h after i.n. infection of B6 WT and CD169-DTR mice (mean±SD, n ≥ 4 mice/group) with Spn. (C) The numbers of BALF AMs, (D) lung bacterial burdens, and (E) the numbers of CD11b+Ly6C+ monocytes (Mo) and CD11b+Ly6G+ neutrophils (PMN) 24 h after i.n. challenge of day seven PR8-infected B6 WT and CD169-DTR mice (mean±SD, n ≥ 5 mice/group) with Spn. Naïve (A-B) or PR8-infected (C-E) mice were treated i.p. with DT (+DT) or PBS control four days before Spn infection. *P<0.05, **P<0.01***P<0.001, Tukey’s multiple comparisons test or t-test (E). Data shown are representative of two independent experiments.