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. Author manuscript; available in PMC: 2021 Sep 2.
Published in final edited form as: ACS Appl Mater Interfaces. 2020 Aug 24;12(35):38950–38961. doi: 10.1021/acsami.0c10276

Figure 3.

Figure 3.

(A) Confocal microscopy of MC internalization by APCs. Representative 3D renderings of z-stacks created in the Imaris microscopy image analysis software displaying MC internalized within BMDCs (A) and RAW 264.7 cells (B). Cells were treated with BODIPY-MC (green) for 24 hours and then stained with CellTracker (blue) and LysoTracker (red). Scale bar is 10 μm. (C) Quantification of MC internalization after 24 hours verified by z-stack. Percent of cells with one or more internalized MC. Data represented as mean ± standard error of the mean. **p < 0.01, ****p < 0.0001.