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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Cell Signal. 2020 Jul 14;74:109713. doi: 10.1016/j.cellsig.2020.109713

Fig. 6. Phosphomimetic substitution at Ser107 inhibits C/EBP-β-induced CHOP nuclear localization.

Fig. 6.

(A) Western blotting for CHOP, C/EBP-β, Lamin-B1, and HSP90 of nuclear and cytoplasmic cell fractions from Vero cultures collected at 24h after acute transfection with WT CHOP, CHOP Ser107Ala, or Ser107Glu with WT C/EBP-β or the control vector (pSG5) at a ratio of 1:4. (B) Densitometry is represented as the ratio of nuclear to cytoplasmic CHOP signal; n=3. (C-D) Western blotting and densitometry of nuclear and cytoplasmic cell fractions from Vero cultures transfected with either WT CHOP or CHOP Ser107Glu with either C/EBP-β or pSG5 at a ratio of 1:4 and 4h after treating cultures with 50nM thapsigargin or DMSO control; n=3. S107E DMSO vs. WT DMSO, # p=0.04. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.