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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Mol Cell Endocrinol. 2020 Jun 27;515:110920. doi: 10.1016/j.mce.2020.110920

Figure 1. Suppression of SRF pathway in brown adipocyte differentiation.

Figure 1.

(A, B) Immunoblot analysis of SRF protein tissue distribution (A), and quantification with normalization to heat shock protein 90 (HSP90) as internal control (B) in 3-weeks old mice (n=3/lane). (C) SRF protein levels in mesodermal lineage cell lines, C2C12 murine myoblasts, HIB1B brown preadipocyte, 3T3-L1 white preadipocytes and C3H10T1/2 mesenchymal stem cells. (D, E) Dynamic protein expression of SRF signaling components and brown adipogenic differentiation markers (C/EBPα, C/EBPβ, Prdm16) (D), and SRF transcriptional targets involved in cytoskeleton organization (smooth muscle α-actin SMA, vinculin and α-tubulin) along the indicated time course of C3H10T1/2 differentiation induced by brown adipogenic cocktail (E), with Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal control. (F) Fluorescence staining of F-actin stress fibers by Alexa 546-conjugated phalloidin and BODIPY 493/503 labeling of lipid droplets before and at day 8 of brown adipogenic differentiation. Arrowheads: Bodipy-stained lipid droplets. Scale bar: 50μm.