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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Mol Cell Endocrinol. 2020 Jun 27;515:110920. doi: 10.1016/j.mce.2020.110920

Figure 5. Loss of SRF or MRTFs induces commitment of mesenchymal stem cells toward brown adipocyte lineage under 5-Azacytidine treatment.

Figure 5.

(A and B) Induction of adipogenic and thermogenic genes in C3H10T1/2 cells with inhibition of SRF (A) or MRTF (B), as assessed by immunoblot analysis during lineage commitment and differentiation induced by 5-Azacytidine (20μM) for 25 days. (C, D) Enhanced lipid accumulation as assessed by Bodipy staining in Srf (C) or Mrtf1/2 knockdown cells (D) at 15 days of azacytidine induction (Scale bar=50μm). (E) RT-qPCR analysis of brown adipocyte lineage markers expression at day 0 and 7 of azacytidine treatment (n=3/group). Data are displayed as mean ± SEM. *P < 0.05, ** P < 0.01, *** P < 0.001.