Skip to main content
. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Stem Cell Rev Rep. 2020 Oct;16(5):946–953. doi: 10.1007/s12015-020-10021-w

Figure 1. Numbers of G-CSF mobilized HSCs and HPCs per ml blood.

Figure 1.

A combination of three independent experiments is shown; each experiment assessed three mice. PB and BM was harvested and processed in a hypoxic chamber (3% O2, 5% CO2, N 2) or ambient air (~21% O2) from G-CSF mobilized C57BL/6 mice, and analyzed for HSC and HPC numbers. Numbers of LT-HSCs (Ai), ST-HSCs (Aii), MPPs (Aiii), CMPs (Aiv), GMPs (Av), MEPs (Avi) and CLPs (Avii) per ml blood were assessed by flow cytometry. A flow cytometry dot plot representative of LT-HSCs, ST-HSC and MPP (Aviii). Progenitor cell numbers and were analyzed using a functional HPC colony assay examining CFU-GM, BFU-E, and CFU-GEMM per ml PB (Bi) and per femoral BM (Bii). Data are presented as mean± SEM. *p < 0.05, **p < 0.05, ***p < 0.005 when analyzed by Student’s t test.