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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Mol Carcinog. 2020 Aug 5;59(10):1116–1128. doi: 10.1002/mc.23241

Figure 5.

Figure 5.

IL-8 is a novel downstream target of FoxQ1 in SUM159 cells. A, RNA-seq analysis for IL-8 gene expression in EV cells and FoxQ1 overexpressing SUM159 cells. Data shown are mean ± SD (n = 3). *P < 0.05 by two-sided Student's t-test. B, Positive correlation between FoxQ1 and IL-8 expression in breast tumor TCGA dataset (n = 1097). Pearson's test was used to determine statistical significance of the correlation. C, Quantification of IL-8 mRNA expression by qRT-PCR in EV and FoxQ1 overexpressing SUM159 cells. Data shown are mean ± SD (n = 3). *P < 0.05 by two-sided Student's t-test. D, Quantification of IL-8 secretion in the media of EV and FoxQ1 overexpressing SUM159 cells. Data shown are mean ± SD (n = 3). *P < 0.05 by two-sided Student's t-test. E, Identification of putative FoxQ1 binding sites at the promoter region of IL-8 in SUM159 cells. The bar graph shows recruitment of FoxQ1 at the IL-8 promoter by ChIP analysis. The results shown are mean ± SD (n = 3). *P < 0.05 by two-sided Student's t-test. Experiments were repeated with similar results.