Skip to main content
. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Neuroscience. 2020 Jul 19;443:30–43. doi: 10.1016/j.neuroscience.2020.07.020

Figure 1. C57BL/6J mice exhibit a significant reduction of microinjected FLsAβ42 and abundant microglial activation 5 days after surgery.

Figure 1.

(A and B) Two hours or five days after surgery, the brains were removed and fixed. Brains were sectioned coronally at 20 μm on a cryostat. Cryosections were blocked and incubated with anti-Iba-1 or anti-CD68 antibodies. Nuclei were visualized by TO Pro-3 staining. Images with microinjected FLsAβ42 in the frontal cortex were obtained using a Zeiss LSM 510 confocal microscope. (B) The mean intensities of microinjected FLsAβ42 2 h and 5 days after surgery were analyzed (n = 5 females per group, 8 sections per mouse). The data, expressed as percentages of control (i.e. 2 h), represent the means ± SEM of 5 microinjected mice (40 sections) from A; ** p < 0.01 versus control (Student’s t-test). Detailed statistical results are shown in Table 2. Scale bar = 50 μm.