. Author manuscript; available in PMC: 2021 Sep 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Jun 23;1152:122254. doi: 10.1016/j.jchromb.2020.122254

Table 2.

Intra-and inter-day precision and accuracy for QC samples of silmitasertib in various matrices (Intra-day: n=6; Inter-day: n=18)

Matrix	Nominal ^a(ng/ml)	Mean conc. ^a(ng/ml)	Within-day (n= 6)		Mean conc. ^a(ng/ml)	Between-day (n= 18)
Matrix	Nominal ^a(ng/ml)	Mean conc. ^a(ng/ml)	%CV	%Nominal	Mean conc. ^a(ng/ml)	%CV	%Nominal
Human plasma (0.2-125 ng/ml)	0.2	0.199	4.3	99.4	0.204	6.3	101.9
	0.6	0.603	5.4	100.5	0.600	4.6	99.9
	7.5	7.57	2.9	100.9	7.32	3.3	97.6
	100	94.6	2.5	94.6	93.6	2.1	93.6
Human plasma (32-20,000 ng/ml)	32	31.66	2.4	98.9	32.76	8.2	102.4
	96	91.93	2.8	95.8	95.28	4.1	99.2
	1300	1260.4	2.5	97.0	1318.8	5.1	101.4
	17,000	15,168.6	1.9	89.2	15,482.8	3.0	91.1
Human CSF	0.2	0.213	1.9	106.3	0.210	5.2	107.0
	0.6	0.590	4.4	98.3	0.610	5.7	101.7
	3	2.890	3.5	96.5	2.93	4.8	97.5
	18	17.55	4.7	97.5	17.34	4.5	96.3
Mouse brain	2	1.73	5.0	86.4	1.82	10.7	91.0
	4	3.58	5.3	89.4	3.84	7.2	96.1
	16	15.01	3.1	93.8	15.92	6.3	99.5
	32	28.70	1.7	89.7	31.34	9.2	97.9
^bPrimate brain	4	4.32	3.2	108.0	4.29	4.4	107.2
^bPrimate brain	32	31.59	2.3	98.7	32.42	5.1	101.3

The unit of concentration is ng/g for QC samples in mouse brain and primate brain

QC samples for primate brain were analyzed by using the calibration curve of mouse brain homogenate.