Table 3.

Matrix factors and recovery in various matrices

	Conc. e(ng/ml)	aMatrix factor				%Recovery
		silmitasertib		bISTD		silmitasertib		bISTD
		cMean	CV%	cMean	CV%	dMean	CV%	dMean	CV%
Human	0.6	1.06	5.7	0.97	2.1	84.2	4.2	87.6	5.3
plasma	100	1.06	1.9	1.00	3.0	77.4	2.0	79.4	3.0
Human	0.6	1.00	5.4	1.01	3.8	103.4	2.9	105.9	1.8
CSF	18	1.00	3.3	0.99	3.2	106.2	1.5	108.5	3.5
Mouse brain	4	0.70	8.3	1.12	9.8	74.1	6.1	83.8	3.5
	32	0.83	3.4	1.12	1.1	67.4	2.4	95.3	4.7

^aMatrix factor (MF) and recovery were evaluated for silmitasertib at two concentration levels for each matrix

^bISTD is at the fixed concentration of 6 ng/ml

^cMean MF of six lots of human plasma and CSF

^dMean recovery from one lot of human plasma and CSF in triplicate

^eFor mouse brain, the concentration unit is ng/g.