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. Author manuscript; available in PMC: 2021 Aug 26.
Published in final edited form as: Cell Syst. 2020 Jul 28;11(2):121–130.e6. doi: 10.1016/j.cels.2020.07.005

Figure 3. Translation elongation kinetics change only with aaRS underproduction.

Figure 3.

(A) Relationship between aaRS production and translation elongation rate to be tested. (B) Simplified schematic of the translation elongation cycle. The step of ternary complex binding (1) depends on aaRS production, but may not be rate-limiting for the elongation cycle. (C) Changes in codon occupancy across aaRS production. Average codon occupancy for indicated codons was acquired by ribosome profiling at the specific aaRS induction levels for LeuS and SerS (STAR methods). 81 genes for LeuS induction and 218 genes for SerS induction passed the depth threshold in all experiments and were used for analysis. (D) Average ribosome occupancy for all 61 sense codons for aaRS overproduction and control. See also Figure S3.