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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Mol Cell. 2020 Jul 1;79(4):588–602.e6. doi: 10.1016/j.molcel.2020.06.010

Figure 6: Disome profiling shows a unique stalling event in ubiquitin coding genes.

Figure 6:

A. Monosome (gray, top) and disome (red, bottom) profiles of the UBI4 gene, which encodes the pro-ubiquitin peptide. Pro-ubiquitin is shown as a bar and the ubiquitin cleavage sites are indicated by dashed lines. Disome peaks at PPD motifs are periodically present downstream of the cleavage sites. Stalling places the cleavage site just outside the peptide exit tunnel, suggesting these sites may be functionally important.

B. Monosome (gray, top) and disome (red, bottom) profiles of RPL40A and RPL40B genes, which both code for translationally fused ubiquitin and eL40 protein. Ubiquitin cleavage sites are shown with dashed lines. Disome peaks at the indicated motifs could be important for co-translational processing of ubiquitin from its fusion with eL40.

See also Figure S6.