| Antibodies |
| Anti-eIF2α |
Custom antibody made in the laboratory of Thomas Dever (NIH) |
N/A |
| Anti-eIF2α-phospho |
Abcam |
32157 |
| Anti-H3 |
Abcam |
1791 |
| Bacterial and Virus Strains |
| NEB 5-alpha Competent E. coli cells |
NEB |
C2987 |
| Chemicals, Peptides, and Recombinant Proteins |
| Cycloheximide |
Sigma-Aldrich |
C7698 |
| 3-Amino-1,2,4-triazole (3-AT) |
Sigma-Aldrich |
A8056 |
| NEBuilder HiFi DNA Assembly Cloning Kit |
NEB |
E5520 |
| Q5 Site-Directed Mutagenesis Kit |
NEB |
E0554S |
| RNase I |
Ambion |
AM2294 |
| Adenylation mix |
NEB |
E2610 |
| PNK |
NEB |
M0201L |
| T4 RNA Ligase 2 Truncated, K227Q |
NEB |
M0351L |
| RecJ exonuclease |
Biosearch technologies |
RJ411250 |
| 5’ deadenylase |
NEB |
M0331S |
| Ribo-Zero Gold rRNA Removal Kit |
Illumina |
MRZY1306 |
| Superscript III Reverse Transcriptase |
Invitrogen |
18080044 |
| CircLigase ssDNA ligase |
Biosearch technologies |
CL4115K |
| Dynabeads MyOne Streptavidin C1 |
Invitrogen |
65001 |
| Phusion DNA Polymerase |
ThermoFisher Scientific |
F530L |
| Critical Commercial Assays |
| High Sensitivity DNA Kit |
Agilent |
5067-4626 |
| Millipore Ziptips |
Milipore |
ZTC18S096 |
| Pierce BCA Protein Assay Kit |
ThermoFisher Scientific |
23225 |
| Deposited Data |
| Raw and processed data, see also Table S3
|
This paper |
GEO: GSE139036
|
| Raw western blot gel images |
This paper, Mendeley data |
http://dx.doi.org/10.17632/53frn8z6xt.1 |
| Experimental Models: Organisms/Strains |
| See Table S1
|
|
|
| Oligonucleotides |
| See Table S2
|
This paper |
N/A |
| Recombinant DNA |
| N/A |
|
|
| Software and Algorithms |
| Scripts used for the analysis |
This paper |
https://github.com/guydoshlab/Yeastcode1 |
| Igor Pro 7 |
Wavemetrics |
15-500 |
| BOWTIE 1.1.2 |
Github |
Langmead et al., 2009 |
| Cutadapt |
|
Martin, 2011 |
| Biopython |
Github |
https://github.com/biopython/biopython |
| BCbio |
Github |
https://github.com/chapmanb/bcbio.variation |
| DESEQ2 |
Bioconductor |
Love et al., 2014 |
| RStudio Version 1.2.5001 |
RStudio, Inc., Boston, MA |
N/A |
| Prism 8 for macOS, version 8.1.0 |
GraphPad Software, Inc. San Diego, California USA |
www.graphpad.com |
| Other |
| 15%TBE-Urea polyacrylamide gel |
Bio-Rad |
3450091 |
| 10% TBE-Urea gel |
Bio-Rad |
3450089 |
| 4–12% Bis-Tris Plus gel |
Invitrogen |
NW04125 |
| 4–20% Mini-Protean TGX gel |
Bio-Rad |
4561096 |
| PVDF membrane pack |
Bio-Rad |
1704156 |
