KEY RESOURCES TABLE
| REAGENT/RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| mouse anti-GluRIIA | DSHB | MH12B; RRID:AB_528269 |
| mouse anti-Dlg | DSHB | 4F3; RRID:AB_528203 |
| mouse anti-Brp | DSHB | Nc82; RRID:AB_2314866 |
| chicken anti-GFP | Abcam | Cat#: ab13970; RRID:AB_300798 |
| mouse anti-α-Tubulin | Sigma-Aldrich | DM1A; RRID:AB_2617116 |
| rat anti-Neto-ex | Custom antibody | N/A |
| rabbit anti-Neto-β1 | Custom antibody | N/A |
| rabbit anti-GluRIIB | Custom antibody | N/A |
| rabbit anti-GluRIIC | Custom antibody | N/A |
| Alexa Fluor 647 goat anti-HRP | Jackson ImmunoResearch Laboratories, Inc | Cat#: 123–605-021; RRID:AB_2338967 |
| Chemicals, Peptides and Recombinant Proteins | ||
| Philanthotoxin-343 | Sigma-Aldrich | Cat#: P206 |
| Oregon-Green 488 BAPTA-1 dextran | ThermoFisher | Cat#: O6798 |
| Dextran, Alexa Fluor 568 | ThermoFisher | Cat#: D22912 |
| SlowFade Gold with DAPI | Invitrogen | Cat#: S36938 |
| ProLong Gold | Invitrogen | Cat#: P36930 |
| Oligonucleotides | ||
| gRNA α−1 sense | 5’P-CTTCGGTTTCTGGGGATAAGATGG | N/A |
| gRNA α−1 antisense | 5’P- AAACCCATCTTATCCCCAGAAACC | N/A |
| gRNA α−3 sense | 5’P- CTTCGGAATATAATGGAAAAATGA | N/A |
| gRNA α−3 antisense | 5’P- AAACTCATTTTTCCATTATATTCC | N/A |
| Neto-F1 | 5’-AGTCCCTTTACCACTCCATTAGCC | N/A |
| Neto-R1 | 5’-TTGCGAGTGCTTTTGCCTGC | N/A |
| CG3822-gATD1 sense | 5’P- CTTCGCATTTTGAATTCGTTCGCGA | N/A |
| CG3822-gATD1 antisense | 5’P- AAACTCGCGAACGAATTCAAAATGC | N/A |
| CG3822-gATD2 sense | 5’P- CTTCGACAGCTTCCATGCCGGGAAA | N/A |
| CG3822-gATD2 antisense | 5’P- AAACTTTCCCGGCATGGAAGCTGTC | N/A |
| CG3822-F1 | 5’-CAAACCCTTGGAGAAATAGGG | N/A |
| CG3822-R1 | 5’-CTACGATTGAGGTCCCCTTG | N/A |
| Software and Algorithms | ||
| Imaris 8.0 | Bitplane | N/A |
| ImageJ 2.0 | National Institutes of Health | N/A |
| Prism 8 | GraphPad Software, Inc. | N/A |