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. 2020 Aug 28;14:233. doi: 10.3389/fncel.2020.00233

Figure 1.

Figure 1

(A) HUES8s organoid at 5 weeks—tile scanning of NISSL staining. Representative images are shown of (A1) control, non-treated organoid, and (A2) Tumor Necrosis Factor-α (TNF-α) (1 ng/ml) treated organoid. (B) Rosette formation in HUES8 organoids at 5 weeks—immunostaining of Ki67+ proliferating cells (green immunostaining) and DAPI stained nuclei (blue). (B1) Control HUES8 organoids not exposed to TNF formed cortical rosettes with Ki67+ proliferative cells. (B2) In HUES8, organoids exposed to 1 ng/ml TNF and (B3) in HUES8 organoids exposed to 10 ng/ml TNF Ki67 positive cells were dispersed throughout the organoids. (C) Neuronal development in HUES8 organoids at 5 weeks, pan-Neu immunostaining—red, DAPI—blue, tile scanning. (C1) In control organoids, pan-Neu+ neurons were located throughout the organoids outside of the ventricular zones (VZ), in the intermediate zone (IZ) and were concentrated in the cortical zone (CZ). (C2) HUES8 organoids exposed to 1 ng/ml TNF exhibited disorganized pan-Neu neuronal development, with clusters of pan-Neu+ cells dispersed throughout the tissue, and concentrated in the scar-like region. Note fewer pan-Neu+ neurons in the CZ of TNF-exposed organoids.