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. 2020 Aug 28;14:233. doi: 10.3389/fncel.2020.00233

Figure 2.

Figure 2

(A) Disorganized migration of proliferating cells in SZ and TNF exposed 5-week control (C) organoids; (A1)—C, (A2)—C+TNF, (A3)—SZ, (A4)—SZ+TNF. The images show organoids were immunostained for Ki67 (red) used to quantify cell densities in cortical regions of interest (ROIs; rectangle), *marks superficial cortical region. In organoids shown in (A2–A4), note the dispersion of proliferating (Ki67+) cells outside the VZ into IZ and CZ. Examples of ROIs—circular—IZ, rectangular—CZ. (B) Changes in ki67+ cell densities. (B1) IZ—Density of Ki67+ cells in IZ was analyzed in circular ROIs [four sections per organoid of each of the four iPSC organoid groups (C, C+TNF, SZ, SZ+TNF), 1–3 ROIs per section]. Bars represent average ROI cell numbers per section. Two-way ANOVA compared—main effect of SZ, F = 10.44, p < 0.005; TNF, F = 3.07, p = 0.08. LSD post hoc analysis: *,***Different from C, p < 0.05, p < 0.0001; ##,###different from C+TNF. (B2) CZ—Density of Ki67+ cells in CZ was analyzed in rectangular ROIs (two to four sections per organoid of each group, three to seven ROI per section). Bars represent average ROI cell numbers per section. Two-way ANOVA—main effects of SZ, F = 40.2, p < 0.0001; no effect of TNF F = 0.139, p = 0.7; no significant interaction F = 2.34. *,***Different from C, p < 0.05, p < 0.0001; ##,###different from C+TNF.