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UMI-Gen’s workflow: Control samples are used to create a background noise frequency matrix and the user provides a CSV file with a list of the wanted variants. Using the FASTA and the BED files, UMI-Gen creates a first set of UMI-tagged reference reads. Artifacts are then inserted to mimic the sequencer’s background noise. Finally, the tool uses the list provided by the user to insert variants at their exact locations.
