FIGURE 3.

Ibrutinib treatment reduces inflammation in the liver of mice fed a via inhibition of NF‐κB and the NLRP3 inflammasome. (a) Representative western blots for phosphorylation of Tyr²²³ on BTK in the liver and normalized to total BTK; for phosphorylation of Tyr¹²¹⁷ on PLCγ in the liver and normalized to total PLCγ and (b) quantified using densitometry. (c) Representative western blots for phosphorylation of Ser^32/36 on IKBα in the liver and normalized to total IKBα; for phosphorylation of Ser^176/180 on IKKα in the liver and normalized to total IKKα; (d) nuclear translocation of p65 and (e) quantified using densitometry. Data shown are individual values with means ± SEM; n = 3–6 per group. ^* P < 0.05, significantly different from HFD + Veh; one‐way ANOVA with Bonferroni post hoc test. (f) Relative gene expression of IL‐1β, IL‐18, TNFα, and IL‐10 were assessed by qPCR and normalized to 18S. (g) Representative western blots for NLRP3 inflammasome assembly, ASC, formation of gasdermin, the proteolytic cleavage of pro‐caspase 1 to caspase 1 and formation of IL‐1β normalized to β‐actin and (h) quantified using densitometry. Data shown are individual values with means ± SEM; n = 6–10 per group. ^* P < 0.05, significantly different from HFD + Veh; one‐way ANOVA with Bonferroni post hoc test