FIGURE 4.

Ibrutinib treatment reduces inflammation in adipose tissue of mice fed a HFD. Relative gene expression of CXCL1, CCL2, and CCL5 were assessed by qPCR and normalized to 18S in epididymal (a) and inguinal (c) adipose tissues. Relative gene expression of CD68 and BTK were assessed by qPCR and normalized to 18S in the liver. Data shown are individual values with means ± SEM. n = 6 per group. ^* P < 0.05, significantly different from HFD + Veh; one‐way ANOVA with Bonferroni post hoc test. Correlation of relative gene expression of CD68 to BTK in epididymal (b) and inguinal (d) adipose tissues. Correlation of BTK and CD68 mRNA levels. Correlation was calculated by linear regression including 95% confidence intervals