FIGURE 6.
Chelation of ionized calcium by SNF472 and by sodium thiosulfate (STS). We added increasing concentrations of SNF472 or STS to a physiological (2.5 mM) calcium solution in 0.15‐M NaCl at pH 7.4 and recorded ionized calcium levels. Chelation is shown as the concentration of ionized calcium in the absence of chelator minus the observed concentration in the presence of chelator. A semi‐logarithmic concentration–response curve was used to determine EC50, hillslope and maximum response (Emax)