Table 2.
Epitopes of the S-protein of human coronaviruses selected for final multi-epitope vaccine construct.
| Epitope type | Coronavirus S-protein | Start residue | End residue | Epitope sequence |
|---|---|---|---|---|
| B-cell epitope | 229E | 1 | 42 | MFVLLVAYALLHIAGCQTTNGLNTSYSVCNGCVGYSENVFAV |
| OC43 | 437 | 590 | LPAANVSVSRFNPSTWNKRFGFIEDSVFKPRPAGVLTNHDVVYAQHCFKAPKNFCPCKLNGSCVGSGPGKNNGIGTCPAGTNY LTCDNLCTPDPITFTGTYKCPQTKSLVGIGEHCSGLAVKSDYCGGNSCTCRPQAFLGWSADSCLQGDKCNI | |
| KU1 | 1151 | 1293 | TSFKTVLVSPGLCLSGDRGIAPKQGYFIKQNDSWMFTGSSYYYPEPISDKNVVFMNSCSVNFTKAPFIYLNNSIPNLSDFEAEF SLWFKNHTSIAPNLTFNSHINATFLDLYYEMNVIQESIKSLNSSFINLKEIGTYEMYVK | |
| NLC3 | 1212 | 1356 | PDYVDVNKTLQEFAQNLPKYVKPNFDLTPFNLTYLNLSSELKQLEAKTASLFQTTVELQGLIDQINSTYVDLKLLNRFENYIK WPWWVWLIISVVFVVLLSLLVFCCLSTGCCGCCNCLTSSMRGCCDCGSTKLPYYEFEKVHVQ | |
| MERS | 172 | 251 | LPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEIL | |
| SARS CoV | 377 | 522 | LCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDIS NVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKN | |
| SARS CoV-2 | 390 | 536 | LCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQ AGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKN | |
| CTL epitopes | OC43 | 953 | 961 | LSENQISGY |
| KU1 | 248 | 256 | NTDNETLEY | |
| NLC3 | 1272 | 1278 | LQINSTY | |
| MERS | 769 | 777 | QVDQLNSSY | |
| SARS CoV | 847 | 855 | LTDDMIAAY | |
| SARS CoV-2 | 865 | 873 | LTDEMIAQY | |
| HTL epitopes | OC43 | 972 | 986 | PPWTAAAGVPFYLNV |
| NLC3 | 940 | 954 | GGLTSAAAIPFSLAL | |
| MERS | 959 | 973 | GWTAGLSSFAAIPFA | |
| SARS CoV | 852 | 866 | IAAYTAALVSGTATA | |
| SARS CoV-2 | 512 | 526 | VLSFELLHAPATVCG |