Fig. 1.

AIBP expression is decreased in glaucomatous retinas and pressure-induced RGCs. (A) Apoa1bp gene expression in control and injured retina at 1 day after acute IOP elevation. N = 4 mice. (B) AIBP protein expression in control and injured retina at 1 day after acute IOP elevation. N = 3 mice. (C) Representative images showed AIBP (green) and TUJ1 (red) immunoreactivities at 1 day after acute IOP elevation. Arrows indicate AIBP immunoreactivity co-labeled with TUJ1 in RGC somas and arrowhead indicates AIBP co-labeled with TUJ1 in RGC axon bundle. (D) AIBP protein expression in control and injured RGCs at 3 day after elevated HP. n = 3 independent experiments with cultures. (E) Representative images showed AIBP (green), TUJ1 (red) and Brn3a (yellow) immunoreactivities. (F) In higher magnification images, arrows indicate AIBP immunoreactivity co-labeled with TUJ1 in RGC somas and arrowhead indicates AIBP co-labeled with TUJ1 in RGC axon bundle. (G) Quantitative fluorescent intensity showed a significant decrease in AIBP immunoreactivity in the inner retina of glaucomatous DBA/2J mice. N = 4 mice. (H) Representative images showed ABCA1 (green), AIBP (red) and Brn3a (yellow) immunoreactivities. Concave arrowheads indicate ABCA1-positive RGCs co-labeled with AIBP and Brn3a. (I) Quantitative fluorescent intensity showed a significant decrease in ABCA1 immunoreactivity in the GCL of glaucomatous DBA/2J mice. N = 4 mice. Error bars represent SEM. Statistical significance determined using Student's t-test. *P < 0.05; **P < 0.01; ***P < 0.001. Blue is Hoechst 33342 staining. Scale bar: 20 μm. CNT, control; GCL, ganglion cell layer; HIOP, high intraocular pressure; EHP, elevated hydrostatic pressure; INL, inner nuclear layer; IPL, inner plexiform layer; N'P, no pressure; ONL, outer nuclear layer; OPL, outer plexiform layer. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)