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. 2020 Sep 10;6:82. doi: 10.1038/s41420-020-00317-8

Fig. 6. miR-185-5p suppresses FADD protein and mRNA expression levels.

Fig. 6

a MLE15 cells were exposed to hyperoxia for 1 day in the presence or absence of miR-185-5p inhibitor. The expression level was measured using real-time PCR. b Live mice were treated with hyperoxia for 0 days, 1 day, 2 days, and 3 days, and RNA was isolated from alveoli tissue. The expression level was measured using real-time PCR. c, d FADD is a direct target of miR-185-5p in both (c) mice and (d) humans. e, f MLE15 cells were transfected with either miR-185-5p mimics or inhibitors, and treated with room air or hyperoxia for 1 day. e Immunofluorescence analysis of phospho-MLKL f Quantified immunofluorescence analysis of relative protein expression. g and h: MLE15 cells were transfected with miR-185-5p mimics and treated with room air or hyperoxia for 1 day, then (d) FADD protein expression was measured by Western blot. e Protein levels were quantified and normalized to actin. The figures represent four independent experiments (n = 4 samples per group) with identical results. Mean ± SD; *p < 0.05, **p < 0.01.