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. 2020 Sep 10;10:14930. doi: 10.1038/s41598-020-71901-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Macrophage and dendritic cell ex vivo verification using TPE-FLIM and bright field microscopy. Side by side comparison of TPE-FLIM images (mean fluorescence lifetime τ_m in the range 300–1,300 ps) (a, c, e) and corresponding light microscopic images (b, d, f) after staining of human skin biopsy cryo-sections for M1 macrophages with CD68 (b), M2 macrophages with CD163 (d) and dendritic cells with CD11c (f) and counterstained nuclei with Mayer's hemalum solution. MCs are marked with orange circles, macrophages and dendritic cells with white/gray circles, respectively. The laser-burned labels are 28 µm × 28 µm. Images have been rotated and zoomed to match the same orientation and size. Scale bar: 30 µm.