Table 1.

TPE-FLIM parameters τ₁, τ₂, τ_m, a₁/a₂ and TPE-AF intensity of the pure and IgE-sensitized resting and activated/degranulated MCs, of the main dermal cells measured in vitro in the cell culture and of the shorter and longer τ_m MCs measured in vivo on the volar forearm of healthy volunteers, allergic and mastocytosis patients and ex vivo in human skin cryo-sections.

	Number of cells	τm in ps	τ1 in ps	τ2 in ps	a1/a2	TPE-AF intensity, photons/mW
Resting MCs	43	1,248 ± 287	533 ± 266	2,289 ± 317	1.5 ± 0.5	1,300 ± 400
Activated/degranulated MCs	13	862 ± 268	288 ± 130	1920 ± 287	2.5 ± 2.0	900 ± 200
IgE-sensitized resting MCs	14	1,107 ± 54	412 ± 83	2,427 ± 256	1,9 ± 0.3	800 ± 80
IgE-sensitized activated/degranulated MCs	15	747 ± 44	217 ± 21	1877 ± 117	2.1 ± 0.6	1,000 ± 120
Macrophages (short lifetime)	34	461 ± 175	225 ± 84	1,289 ± 278	4.8 ± 3.4	3,000 ± 500
Macrophages (long lifetime)	20	1,348 ± 188	1,016 ± 220	2,432 ± 239	2.6 ± 1.0	800 ± 200
Dendritic cells	14	1,265 ± 180	434 ± 188	2,578 ± 328	1.6 ± 0.2	538 ± 258
Fibroblasts	6	921 ± 81	429 ± 51	1983 ± 137	0.5 ± 0.1	469 ± 137
Neutrophils	21	1,074 ± 109	714 ± 250	1795 ± 600	1.5 ± 0.5	500 ± 115
In vivo (healthy skin) MCs (longer τm)	71	1,171 ± 117	344 ± 49	2,301 ± 77	1.5 ± 0.3	300 ± 40
In vivo (healthy skin) MCs (shorter τm)	48	678 ± 151	241 ± 59	1947 ± 230	3.0 ± 1.1	800 ± 100
In vivo (mastocytosis skin) MCs (longer τm)	9	1,211 ± 130	360 ± 76	2,243 ± 141	1.3 ± 0.2	280 ± 40
In vivo (mastocytosis skin) MCs (shorter τm)	3	597 ± 136	173 ± 31	1902 ± 206	3.5 ± 0.8	700 ± 100
In vivo (allergy skin) MCs (longer τm)	8	970 ± 157	310 ± 58	2,204 ± 103	1.8 ± 0.7	320 ± 120
In vivo (allergy skin) MCs (shorter τm)	9	546 ± 89	185 ± 20	1925 ± 147	4.6 ± 1.1	840 ± 160
Ex vivo (skin biopsy) MCs (longer τm)	9	1,204 ± 97	417 ± 86	2,181 ± 166	1.7 ± 1.0	1,600 ± 300
Ex vivo (skin biopsy) MCs (shorter τm)	4	730 ± 140	243 ± 43	1743 ± 167	2.4 ± 0.7	2000 ± 300