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. 2020 Sep 10;11:4535. doi: 10.1038/s41467-020-18237-7

Fig. 6. CLSM images confirm the uptake of nanoparticles.

Fig. 6

a, b Orthogonal views of 3D stacks of CLSM images of SNP–corona complexes confirm the uptake of nanoparticles in THP-1 macrophages (a) and hCMEC/D3 cells (b) in RPMI containing BSA. c, d Orthogonal views of 3D stacks of CLSM images of PsNP–corona complexes confirm the uptake of nanoparticles in THP-1 macrophages (c) and hCMEC/D3 cells (d) in RPMI containing BSA. The images demonstrate that by vigorous washing of nanoparticles, all noninternalized particles had been removed from the cell surface prior to flow cytometry analysis, and they are internalized by both cell types. In CLSM images, no differences in the localization of NPs inside the cells were observed. The cells grown on collagen-coated coverslips were fixed, and then the cell nuclei (blue) and the actin filaments (green color) were stained with Hoechst and phalloidin, respectively. The FITC -labeled nanoparticles are shown in red color. Scale bar, 20 µm. The CLSM analysis was repeated three times independently with similar results. Source data are provided as a Source data file.