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. 2020 Sep 11;9(9):81. doi: 10.1038/s41389-020-00265-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic representation of the method of ECSC isolation. b Morphology of ECSCs isolated from different EC cell lines and human samples on days 1, 3, 5 and 8 (scale bar = 25 μm). c The numbers of spheres produced from different cells were analysed. d qRT-PCR analysis of stemness genes (NANOG, SOX2 and CD133) in ECSC^isk. Data are shown as the mean ± SEM (N = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. Day 1. e Immunoblotting findings of ALDH1 and SOX2 in ECSC^isk on days 1, 2, 4, 5 and 8.