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. 2020 Sep 11;9(9):81. doi: 10.1038/s41389-020-00265-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Morphology of ECSC^isk and ECSC^ecc and immunofluorescent staining of ALDH1 and SOX2. b FACS detection of ALDH1 and CD133 in ECSC^isk and ECSC^ecc. c qRT-PCR analysis of stemness genes (ALDH1, CD133, OCT4, SOX2 and NANOG) in ECSC^isk and ECSC^ecc. d SOX2 protein expression by western blot in different ECSCs and control cells (ISK). e EMT gene expression in ECSC^isk. f FACS data of the percentages of cells positive for ALDH1 and CD133 in two human samples and ECSC^isk. g Statistics of the percentage of CD133-positive cells in ECSC^isk. Mean ± SEM (N = 3). *^/#P < 0.05, **^/##P < 0.01, and ***^/###P < 0.001.