Skip to main content
. 2020 Sep 11;9(9):81. doi: 10.1038/s41389-020-00265-z

Fig. 4. Hypoxia promotes a stem-like state in ECSCs.

Fig. 4

a, b Images showing the morphology (a) and sphere numbers (b) of ECSCisk supplemented with CoCl2 (30 and 100 µM) or KC7F2 (1 and 10 µM). c, d Images showing the morphology of ECSCisk, immunofluorescent staining of SOX2 (c) and the number of ECSCisk colonies (d) subjected to 2 or 21% O2 with or without growth factors. e mRNA expression analysis of HIF-1α and 2α and stemness genes (ALDH1, CD133, OCT4, SOX2 and NANOG) in ECSCisk treated with CoCl2 (100 µM) or KC7F2 (10 µM) under normoxic or hypoxic conditions for 3 days. f, g qPCR (f) and immunoblotting (g) analysis of SOX2 after transfection with an siRNA. h Morphology pictures of ECSCisk after 2 days of SOX2 inhibition in normoxic conditions (scale bar = 25 μm). i The ECSCisk spheres with different SOX2 levels in hypoxic or normoxic conditions. Mean ± SEM. */#P < 0.05, **/##P < 0.01, and ***/###P < 0.001.