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. 2020 Sep 11;9(9):81. doi: 10.1038/s41389-020-00265-z

Fig. 6. Knockdown of ALKBH5 decreases the stem-like state of ECSCs.

Fig. 6

a, b qPCR (a) and immunoblotting (b) analysis of ALKBH5 expression in ECSCisk that were not passaged on days 1, 3, 5 and 8. The medium was changed every day. c, d After HIF overexpression or knockdown, the protein (c) and mRNA (d) levels of ALKBH5 were analysed. e ALKBH5 and HIF protein expression after ALKBH5, HIF-1α, or HIF-2α inhibition. f HIF-1α, HIF-2α, and ALKBH5 protein levels were detected in ECSCisk in different co-transfection groups. g Images show the morphology, and confocal images reveal SOX2 in cells treated with the indicated siRNA or shRNA, supplemented with CoCl2 (100 µM) or KC7F2 (100 µM), or transfected with vector that overexpressed ALKBH5 (scale bar = 25 μm). h ECSCs under different treatments as shown were exposed to 21 or 2% O2, and the number of spheres was determined. i Immunoblotting results for HIF-1α, HIF-2α, ALKBH5, and SOX2 in ECSCisk with ALKBH5, HIF-1α, or HIF-2α inhibition. Mean ± SEM (N = 3). **P < 0.01, ***P < 0.001; #P < 0.05, and ###P < 0.001.