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. 2020 Jun 12;111(7):2248–2258. doi: 10.1111/cas.14490

FIGURE 2.

FIGURE 2

Effects of CP treatment on the proportions of MDSC subsets in spleens of CT26‐bearing mice. A, On day 12 after CT26 inoculation, mice received ip injection of CP (100 mg/kg). Simultaneously, oral supplementation of l‐arginine (30 mg/mouse) was initiated. On day 18, spleens were harvested and cells were enumerated (n = 4). B, Staining strategy for MDSC subsets. C, Proportions of CD11b+ cells. On day 18, the spleens were harvested and stained with the indicated antibodies. D, A representative result is shown. Proportions (E) and numbers (F) of MDSCs in spleen. (n = 4). Data are means ± standard deviations. **P < .01, *P < .05, n.s., not significant (ANOVA)