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. 2020 Aug 28;11:2085. doi: 10.3389/fmicb.2020.02085

FIGURE 1.

FIGURE 1

Time course of biofilm biomass and metabolic activity of two Staphylococcus aureus strains. The biofilm biomass was evaluated using the crystal violet staining method (OD 590 nm, bars) and metabolic activity was determined using the XTT reduction assay (OD 450 nm, lines with filled circles). (A,C) Methicillin-sensitive Staphylococcus aureus (MSSA) ATCC 6538 and methicillin-resistant S. aureus (MRSA) ATCC 43300 were incubated for 96 h and the biofilm biomass and metabolic activity were assessed at selected time points without changing the spent medium or (B,D) renewing the medium every 24 h (MR = Medium Renewal). Data are expressed as the means ± SEM of three independent experiments in triplicate. Statistically significant differences were tested with ordinary one-way ANOVA or Kruskal–Wallis test. *p < 0.05 biofilm biomass vs biofilm biomass at 24 h. #p < 0.05 biofilm biomass vs biofilm biomass at 96 h, ‘p < 0.05 biofilm metabolic activity vs biofilm metabolic activity at 24 h. +p < 0.05 biofilm metabolic activity vs biofilm metabolic activity at 48 h.