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. Author manuscript; available in PMC: 2020 Sep 11.
Published in final edited form as: Atherosclerosis. 2019 Jan 30;282:121–131. doi: 10.1016/j.atherosclerosis.2019.01.023

Fig. 5.

Fig. 5.

FAM220A is directly regulated by miR-92a-3p in human endothelial cells. (A) HCAEC FAM220A mRNA levels with miRNA inhibition; LNA-Scrambled (Scr), LNA-92a, or LNA-489. N = 9. (B) HCAEC FAM220A mRNA levels with miRNA over-expression; miR-92a-3p mimetics or siRNA FAM220A control. N = 8–12. (C) Gene reporter (luciferase) assays with dual transfection of miR-92a-3p mimics and luciferase reporter plasmids harboring the full-length human FAM220A 3′ UTR or mutated FAM220A with a 2 bp deletion in miR-92a-3p target site in HEK293 cells. N = 7. (D–G) Western blots and quantification for STAT3 Y705 phosphorylation, total STAT3, and GAPDH. (D–E) Over-expression of miR-92a-3p (mimetics) in HCAEC in growth factor-free culture media. N = 8. (F and G) Over-expression of FAM220A in HCAEC. N = 3. For comparisons between 2 groups Mann-Whitney non-parametric tests were used, and when comparing between > 2 groups One-way ANOVA tests were used with Bonferroni corrections, α = 0.025 for 2 comparisons and α = 0.017 for 3 comparisons.