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. 2020 Aug 28;11:2158. doi: 10.3389/fimmu.2020.02158

FIGURE 2.

FIGURE 2

Ibrutinib alters the inflammatory profile of macrophage population. NLC were treated overnight with ibrutinib and then stimulated with A. fumigatus conidia or zymosan. (A) Bar diagrams show the expression level of TNF-α and IL-1β in NLC measured by real-time PCR. As shown ibrutinib decreased the amount of TNF-α and IL-1β in presence or not of A. fumigatus stimulation (n = 8, *P < 0.05, **P < 0.01). (B) Ibrutinib was able to reduce the expression of both TNF-α and IL-1β in NLC with or without zymosan stimulation (n = 8, *P < 0.05, **P < 0.01). (C) Dot plot diagram depicts the ability of NLC to secrete TNF-α after an overnight incubation with ibrutinib and then stimulated or not with A. fumigatus stimulation, measured by CSA. As shown, the level of TNF-α production after treatment with ibrutinib either in presence of A. fumigatus stimulation was significantly decreased (n = 6, *P < 0.05, **P < 0.01). (D) NLC were treated overnight with acalabrutinib and then stimulated with A. fumigatus conidia. Bar diagrams show the relative expression of TNF-α and IL-1β in NLC after treatment either in presence or not of stimulation (n = 6, *P < 0.05, **P < 0.01).